Wade Van Horn
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Phone: 480-965-8322
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ISTB1 402 ISTB1 TEMPE, AZ 85287
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Mail code: 6401Campus: Tempe
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Wade Van Horn is an assistant professor in the School of Molecular Sciences and is an investigator with the Biodesign Institute's Center for Personalized Diagnostics, and the Magnetic Resonance Research Center. He joined Arizona State University in 2012 after an American Heart Association postdoctoral fellowship at Vanderbilt University School of Medicine in the Department of Biochemistry and the Center for Structural Biology. He received his Ph.D. from the University of Utah's Department of Chemistry. His current interests focus on the interplay between biomolecular function and structure, especially as it relates to human physiology and pathophysiology.
Ph.D. Chemistry, University of Utah 2007
Our research interests focus on the roles of membrane proteins in human health and disease, with a particular interest in membrane proteins with therapeutic potential. Membrane proteins, as a class, make up the majority of therapeutic targets and play essential roles in biology and pathophysiology. We investigate the structure, dynamics, and function of this class of proteins with an interdisciplinary approach synthesizing the output from multiple disparate techniques which allows us to tackle challenging yet biomedically relevant problems. Our investigations focus on the following three themes:
1) TRP channel gating and modulation. TRPM8 functions as the primary cold sensor in humans where it integrates, thermo-, chemical-, and voltage-dependencies and is modulated by other proteins and lipids. This channel has significant therapeutic potential in diverse and important diseases such as cancer, chronic pain, obesity, and diabetes. We seek to understand the mechanism of how TRPM8 integrates distinct stimuli and modulators in an effort to unlock the therapeutic potential of TRPM8.
2) Membrane protein structural enzymology. Our lab focuses on two membrane enzymes; vitamin K epoxide reductase (VKOR) and undecaprenol kinase (UDPK). VKOR is the target of the popular anticoagulant warfarin (also Coumadin®) and is one of the hallmark targets of personalized medicine. Undecaprenol kinase is a known virulence factor in gram-positive bacteria and an excellent target for narrow spectrum antibiotic development. For these enzymes we seek to understand the relationship between structure, function, and dynamics.
3) Methods Development for membrane protein structural biology. In an effort to push the boundaries of membrane protein structural biology, we are exploring the use of new, and fine-tuning existing membrane mimics that are compatible with solution NMR. Of particular interest is optimizing reverse micelles as hosts for membrane proteins which have the particular advantage of significantly increasing the size and complexity of NMR-accessible membrane targets.
To accomplish these goals, our research program relies on a number of interdisciplinary techniques including nuclear magnetic resonance spectroscopy (NMR), electrophysiology, enzymology, and computational structural biology.
"Recombinant SARS-CoV-2 envelope protein traffics to the trans-Golgi network following amphipol-mediated delivery into human cells," Hutchison, J.M., Capone, R., Luu, D.D., Shah, K.H., Hadziselimovic, A., Van Horn, W., Sanders, C.R. 297, 2, 100940 (2021).
"Structural and in Vitro Functional Characterization of a Menthyl TRPM8 Antagonist Indicates Species-Dependent Regulation," Journigan, V.B., Alarcón-Alarcón, D., Feng, Z., Wang, Y., Liang, T., Dawley, D.C., Ruhul Amin, A.R.M., Montano, C., Van Horn, W.D., Xie, X-Q, Ferrer-Montiel, A., Fernández-Carvajal, A., 12, 5, 758-767 (2021).
"Hinge-shift mechanism as a protein design principle for the evolution of β-lactamases from substrate promiscuity to specificity," Modi, T., Risso, V.A., Martinez-Rodriguez, S., Gavira, J.A., Mebrat, M.D., Van Horn, W.D., Sanchez-Ruiz, J.M., Banu Ozkan, S., Nature Communications 12, 1 (2021)
"Evidence that the TRPV1 S1-S4 membrane domain contributes to thermosensing," Kim, M., Sisco, N.J., Hilton, J.K., Montano, C.M., Castro, M.A., Cherry, B.R.; Levitus, M., Van Horn, W.D., Nature Communications 11, 1 (2020)
"Hyperthermia induced by transient receptor potential vanilloid-1 (TRPV1) antagonists in human clinical trials: Insights from mathematical modeling and meta-analysis," Garami, A., Shimansky, Y.P., Rumbus, Z., Vizin, R.C. L., Farkas, N., Hegyi, J., Szakacs, Z., Solymar, M., Csenkey, A., Chiche, D.A., Kapil, R., Kyle, D.J., Van Horn, W.D., Hegyi, P., Romanovsky, A.A., Pharmacology & Therapeutics 208 (2020)
"Characterization of HTRPM8 Conformational Dynamic Utilizing Solution NMR," Mebrat, M., Hilton, J.K., Morelan, D., Van Horn, W.D., Biophysical Journal 118 413A-413A (2020)
"Contributions of the Transmembrane Domain to Heat Activation of Human TRPV1," Pratt, A.M., Luu, D., Kim, M., Van Horn, W.D., Biophysical Journal 118 411A-411A (2020)
"Mechanistic and Structural Studies of PIRT Regulation of TRPM8," Van Horn, W..D.; Luu, D.D., Kim, M., Hilton, J.K., Montano, C., Biophysical Journal 118, 413A-414A (2020)
"PIRT the TRP Channel Regulating Protein Binds Calmodulin and Cholesterol-Like Ligands," Sisco, N.J., Luu, D.D., Kim, M., Van Horn, W.D., Biomolecules 10 (2020)
"Structure-Based Design of Novel Biphenyl Amide Antagonists of Human Transient Receptor Potential Cation Channel Subfamily M Member 8 Channels with Potential Implications in the Treatment of Sensory Neuropathies," Journigan, V.B., Feng, Z., Rahman, S., Wang, Y., Amin, A.R.M.R., Heffner, C.E., Bachtel, N., Wang, S., Gonzalez-Rodriguez, S., Fernandez-Carvajal, A., Fernandez-Ballester, G., Hilton, J.K., Van Horn, W.D., Ferrer-Montiel, A., Xie, X.-Q., Rahman, T., ACS Chemical Neuroscience 11, 268-290 (2020)
"Isolation of Functional Temperature Activated Transmembrane Domain of Human TRPM8," Luu, D, Chiu, P-L, Van Horn, WD, Biophysical Journal 118 413A-413A (2020)
"Competitive Interactions between PIRT, the Cold Sensing Ion Channel TRPM8, and PIP2 Suggest a Mechanism for Regulation," Sisco, N.J., Helsell, C.V. M., Van Horn, W.D., Scientific Reports 9 (2019)
"Structural and Evolutionary Insights Point to Allosteric Regulation of TRP Ion Channels," Hilton, J.K.; Kim, M.; Van Horn, W.D., Accounts of Chemical Research 52 (6) 1643-1652 (2019)
"Phosphoinositide lipid interaction studies between TRPM8 and PIRT membrane proteins suggest a lipid shuttling-sequestering regulatory mechanism," Van Horn, W., Abstracts of Papers of the American Chemical Society 257 (2019)
“New Pieces to an Old Puzzle: Identifying the Warfarin-binding Site that Prevents Clotting,” Hilton JK, Van Horn WD, Nature Structure Molecular Biology 24 5-6 (2017)
“A Parallel Stranded G-quadruplex Composed of Threose Nucleic Acid (TNA),” Liao JY, Anosova I, Bala S, Van Horn WD, Chaput JC, Biopolymers 10 e22999 (2017)
“The structural diversity of artificial genetic polymers,” Anosova I, Kowal, EA, Dunn MR, Chaput JC, Van Horn WD, Egli M, Nucleic Acids Res 44 1007-1021 (2016)
“Structural Basis for KCNE3 Modulation of Potassium Recycling in Epithelia,” Kronce BM, Van Horn WD*, Smith J, Kang C, Welch RC, Song Y, Nannemann DP, Sisco NJ, George AL, Meiler J, Vanoye CG, Sanders CR, Science Advances 2 e1501228 (2016)
“Structural Insights into Conformational Differences between DNA/TNA and RNA/TNA Chimeric Duplexes,” Anosova I, Kowal EA, Sisco NJ, Sau S, Liao J, Bala S, Rozners E, Egli M, Chaput JC, Van Horn WD, ChemBioChem 17 1705-1708 (2016)
“Implications of Human Transient Receptor Potential Melastatin 8 (TRPM8) Channel Gating from Menthol Binding Studies of the Sensing Domain,” Rath P, Hilton JK, Sisco NH, Van Horn WD, Biochemistry 55 114-124 (2016)
“Understanding Thermosensitive Transient Receptor Potential Channels as Versatile Polymodal Cellular Sensors,” Hilton JK, Rath P, Helsell CV, Beckstein O, Van Horn WD, Biochemistry 54 2401-2413 (2015)
“NMR Structure of Francisella tularensis Virulence Determinant Reveals Structural Homology to Bet v1 Allergen Proteins,” Zook, J., Mo, G, Sisco, NJ, Craciunescu, FM, Hansen, DT, Baravati, B, Cherry, B, Sykes, K, Wachter, R, Van Horn, WD, Fromme, P., Structure 23 1116-1122 (2015)
"Bicelles at low concentrations," Z. Lu, W.D. Van Horn, J. Chen, S. Mathew, R. Zent, C.R. Sanders, Mol. Pharm. 336 752-761 (2012)
"The amyloid precursor protein has a flexible transmembrane domain and binds cholesterol," P.J. Barrett, Y. Song, W.D. Van Horn, E.J. Hustedt, J.M Schafer, A. Hadziselimovic, A.J. Beel, C.R. Sanders, Science 336 1168-1171 (2012)
"Prokaryotic Diacylglycerol Kinase and Undecaprenol Kinase," W.D. Van Horn, C.R. Sanders, Annu. Rev. Biophys. 41 61-81 (2012)
"Working model for the structural basis for KCNE1 modulation of the KCNQ1 potassium channel," W.D. Van Horn, C.G. Vanoye, C.R. Sanders,Curr. Opin. Struct. Biol. 21 283-291 (2011)
"Functional Delivery of a Membrane Protein into Oocyte Membranes Using Bicelles," C. Kang, C.G. Vanoye, R.C. Welch, W.D. Van Horn, C.R. Sanders, Biochemistry 49 653-655 (2010)
"The Impact of Window Functions on NMR-Based Paramagnetic Relaxation Enhancement Measurements in Membrane Proteins," W.D. Van Horn, A.J. Beel, C. Kang, C.R. Sanders, Biochim. Biophys. Acta 1798 140-149 (2010)
"Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins," H. Kim, S.C. Howell, W.D. Van Horn, Y.H. Jeon, C.R. Sanders, Prog. Nucl. Magn. Reson. Spectrosc. 55 335-360 (2009)
"Reverse Micelle Encapsulation as a Model for Intracellular Crowding," W.D. Van Horn, M.E. Ogilvie, P.F. Flynn, J. Am. Chem. Soc. 131 8030-8039(2009)
"NMR Solution Structure of the Membrane-Integral Diacylglycerol Kinase," W.D. Van Horn, H. Kim, C.D. Ellis, A. Hadziselimovic, E.S. Sulistijo, M.D. Karra, C. Tian, F.D. Sönnichsen, C.R. Sanders, Science 324 1726-1729 (2009)
"Use of reverse micelles in membrane protein structural biology," W.D. Van Horn, M.E. Ogilvie, P.F. Flynn, J. Biomol. NMR 40 203-211 (2008)
"NMR Studies of Encapsulated Macromolecules," P.F. Flynn, A.K. Simorellis, W.D. Van Horn, Annual Reports in NMR Spectroscopy 62 179-219(2007)
"Dynamics of Low Temperature Induced Water Shedding from AOT Reverse Micelles," A.K. Simorellis, W.D. Van Horn, P.F. Flynn, J. Am. Chem. Soc. 128 5080-5090 (2006)
"Low Temperature Studies of Encapsulated Proteins," W.D. Van Horn, A.K. Simorellis, P.F. Flynn, J. Am. Chem. Soc. 127 13553-13560 (2005)
"Phosphoinositide-interacting regulator of TRP (PIRT) has opposing effects on human and mouse TRPM8 ion channels," J.K. Hilton, T. Salepour, N.J. Sisco, P. Rath, W.D. Van Horn*. Journal of Biological Chemistry 293 9423-9434 (2018)
- Van Horn,Wade Dale*, Van Horn,Wade Dale*. PIRT Family Modulation of TRPM8. HHS-NIH-NIGMS(7/1/2015 - 6/30/2020).
- Chaput,John C*, Allen,James Paul, Van Horn,Wade Dale. The Emergence of Protein Folds. NASA-GODDARD SPACE FLIGHT CTR(6/10/2014 - 6/9/2017).
- Chaput,John C*, Van Horn,Wade Dale. Rapid Synthesis And Evolution Of Artificial TNA Polymers Having Affinity And Catalytic Activity. DOD-DARPA(6/1/2014 - 8/31/2017).
- Van Horn,Wade Dale, Webber,Andrew Neil. 2012 Bisgrove Scholar Program: Yr 1 (Post Doc: Melanie Channon). SFAz(7/1/2012 - 12/31/2014).
Courses
2025 Spring
Course Number | Course Title |
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MBB 493 | Honors Thesis |
BCH 463 | Biophysical Chemistry |
2024 Fall
Course Number | Course Title |
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BCH 392 | Intro to Research Techniques |
BCH 492 | Honors Directed Study |
BCH 493 | Honors Thesis |
CHM 392 | Intro to Research Techniques |
CHM 492 | Honors Directed Study |
MBB 492 | Honors Directed Study |
MBB 495 | Undergraduate Research |
CHM 493 | Honors Thesis |
BCH 392 | Intro to Research Techniques |
BCH 598 | Special Topics |
BCH 494 | Special Topics |
CHM 392 | Intro to Research Techniques |
2024 Spring
Course Number | Course Title |
---|---|
MBB 493 | Honors Thesis |
BCH 463 | Biophysical Chemistry |
BCH 494 | Special Topics |
BCH 598 | Special Topics |
2023 Fall
Course Number | Course Title |
---|---|
MBB 492 | Honors Directed Study |
MBB 495 | Undergraduate Research |
BCH 561 | Adv Topics in Biochemistry |
2023 Spring
Course Number | Course Title |
---|---|
MBB 493 | Honors Thesis |
BCH 463 | Biophysical Chemistry |
BCH 598 | Special Topics |
BCH 494 | Special Topics |
2022 Fall
Course Number | Course Title |
---|---|
MBB 492 | Honors Directed Study |
MBB 495 | Undergraduate Research |
BCH 598 | Special Topics |
BCH 494 | Special Topics |
2022 Spring
Course Number | Course Title |
---|---|
MBB 493 | Honors Thesis |
BCH 501 | Current Topics in Biochemistry |
BCH 598 | Special Topics |
BCH 494 | Special Topics |
2021 Fall
Course Number | Course Title |
---|---|
MBB 492 | Honors Directed Study |
MBB 495 | Undergraduate Research |
BCH 501 | Current Topics in Biochemistry |
BCH 598 | Special Topics |
BCH 494 | Special Topics |
2021 Spring
Course Number | Course Title |
---|---|
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
MBB 493 | Honors Thesis |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
BCH 367 | Elementary Biochemistry Lab |
2020 Fall
Course Number | Course Title |
---|---|
MBB 492 | Honors Directed Study |
MBB 495 | Undergraduate Research |
BCH 561 | Adv Topics in Biochemistry |
2020 Spring
Course Number | Course Title |
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BIO 493 | Honors Thesis |
MBB 493 | Honors Thesis |
BCH 501 | Current Topics in Biochemistry |
CHM 598 | Special Topics |
BCH 598 | Special Topics |
BCH 494 | Special Topics |
CHM 494 | Special Topics |
2019 Fall
Course Number | Course Title |
---|---|
MBB 492 | Honors Directed Study |
MBB 495 | Undergraduate Research |
BCH 561 | Adv Topics in Biochemistry |