D. Mitchell Magee joined the Virginia G Piper Center for Personalized Diagnostics as part of a team of scientists and engineers to assist with discovery of high throughput methods for analysis of protein-protein interaction and biomarker discovery. He attended the University of Texas Medical Branch and obtained a B.S. in Medical Technology. After several years’ experience in clinical and research laboratories, he matriculated to Texas A&M University and obtained a Ph.D. in Microbiology. His post-doctoral fellowship was performed at the University of Pittsburgh. He has been working extensively in interdisciplinary teams for high-throughput sample handling and analysis efforts. He has more than 20 years’ experience in studying Coccidioides immunobiology including growth and extraction of fungal extracts, recombinant cloning of immunodominant antigens and evaluating the protective immune response in animal models of coccidioidomycosis. He helped initiate one of laboratories as part of the CDC’s National Tuberculosis Genotyping and Surveillance Network in which we performed RFLP typing of clinical isolates of Mycobacterium tuberculosis. Here we catalogued information on over 5,000 clinical tuberculosis isolates and established and validated DNA extraction and DNA fingerprinting analyses protocols. Additional experience with high throughput functional studies occurred in several projects of vaccine candidate discovery in models of coccidioidomycosis, smallpox, and glanders. These studies combined efforts of bioinformatics, molecular biologists and biologists to identify immunodominant proteins functionally through their protective effects against microbial challenge. Most recently, he has utilized protein microarrays to probe the immune response of patients or animal models of disease to identify immunodominant proteins in patients or animals during disease progression. These large-scale studies of gene expression, peptide and protein microarrays, provide a firm foundation for high throughput studies with an emphasis on developing and refining sample preparation and analysis protocols.
Alexandre Borovkov, D. Mitchell Magee, Andrey Loskutov, Jose Cano Buendia, Cheryl Selinsky, Preston Hunter, C. Rick Lyons, and Kathryn Sykes. New orthopoxvirus vaccine candidates by functionally screening 2 a synthetic gene library for protective antigens. Journal of Virology (2009).
Dewey Magee, R Friedberg, M Woitaske, S Johnston, R Cox. Role of B cells in vaccine-induced immunity against coccidioidomycosis. Infect. Immun (2005).
S Awasthi, V Awasthi, Dewey Magee, J Coalson. Efficacy of Antigen 2/Proline-Rich Antigen cDNA-Transfected Dendritic Cells in Immunization of Mice against Coccidioides posadasii. J. Immunol (2005).
R Cox, Dewey Magee. Coccidioidomycosis: Host Response and Vaccine Development. Clin. Micro. Rev. Clin Microbiol Rev (2004).
S Awasthi, Dewey Magee. Differences in expression of cell surface co-stimulatory molecules, Toll-like receptor genes and secretion of IL-12 by bone marrow-derived dendritic cells from susceptible and resistant mouse strains in response to Coccidioides posadasii. Cell. Immunol (2004).
S Awasthi, Dewey Magee, J Coalson. Coccidioides posadasii infection alters the expression of pulmonary surfactant proteins (SP)-A and SP-D. Respir. Res (2004).
D Ivey, Dewey Magee, M Woitaske, Stephen Johnston, R Cox. Identification of a protective antigen of Coccidioides immitis by expression library immunization. Vaccine (2003).
C Jiang, Dewey Magee, F Ivey, R Cox. Signal sequence of Coccidioides immitis Antigen 2-induced protection against experimental coccidioidomycosis. Infect. Immun (2002).
. . Human Fungal Pathogens (2002).
. . Fungal Pathogenesis: Principles and Clinical Applications (2001).
Research Activity
Magee,Dewey Mitchell*, Haydel,Shelley, Labaer,Joshua. Does Mycobacterium tuberculosis use cellular modiFICations to survive in human macrophages. ASU FDN(7/20/2015 - 7/19/2016).
Wang,Shaopeng*, Magee,Dewey Mitchell, Tao,Nongjian, Wiktor,Peter Jan. An Integrated Microarray Printing and Detection System. (4/1/2015 - 3/31/2016).
Magee,Dewey Mitchell*. Design and Creation of a Registry for Housing Standard Procurement Operating Procedures for Tissue Collection. GBSI(12/22/2014 - 12/21/2016).
Chaput,John C*, Labaer,Joshua, Magee,Dewey Mitchell, Qiu,Ji. A Pipeline for Production of Bivalent Synthetic Antibodies to the Human Proteome. HHS-NIH-NIDDK(9/25/2011 - 7/31/2014).
Magee,Dewey Mitchell*, Labaer,Joshua, Wallstrom,Garrick L. Antibody Biomarkers for Early Detection of Tuberculosis. HHS-NIH-NIAID(8/15/2011 - 7/31/2014).
Magee,Dewey Mitchell*, Labaer,Joshua. Discovery Platform for Cancer Antigens. U of Conn Health Center(9/16/2010 - 8/31/2013).
Labaer,Joshua*, Cheatham,Robert Lee, Dinu,Valentin, Dinu,Valentin, Magee,Dewey Mitchell, Wallstrom,Garrick L, Wallstrom,Garrick L. Integrated Biodosimetry System (IBiS) for High Throughput Medical Care After Radiologic and Nuclear Events. BARDA(12/17/2009 - 1/31/2016).
Johnston,Stephen Albert*, Magee,Dewey Mitchell, Stafford,Phillip. Peptide arrays for antigen identification and dianosis. UTMB(3/1/2008 - 2/28/2009).
Johnston,Stephen Albert*, Magee,Dewey Mitchell, Magee,Dewey Mitchell, Sykes,Kathryn. Tularemia Vaccine Development Team. UNIV OF NEW MEXICO(3/2/2006 - 12/31/2011).
Johnston,Stephen Albert*, Magee,Dewey Mitchell. Integration of Oncogenic Networks in Cancer Phenotypes. DUKE INSTITUTE/GENOME SCIENCES(6/1/2005 - 9/29/2009).
Johnston,Stephen Albert*, Magee,Dewey Mitchell. Region VI Center for Biodefense and Emerging Infections. UNIV OF TEXAS-GALVESTON(3/1/2005 - 2/29/2008).
